The extensive distance between centres and communities in the Kimberley places a high importance on the regional road network. An extensive network of over 7,700 kilometres of roads services the Region's towns, communities and resource development projects. The Great Northern Highway provides a sealed link across the Region. It extends from the North West Coastal Highway in the Pilbara and connects to the Northern Territory, linking the largest regional centres.

Passenger coach services operate along the Great Northern Highway. Comprehensive road freight services are provided by a number of transport companies with a delivery time ranging from Perth to Broome of two to three days and Perth to Kununurra of three to four days. Depot facilities are located in all Kimberley towns. The pastoral industry is heavily reliant upon the Region's road systems to transport approximately 130,000 head of cattle to regional ports each year.

Heavy rains associated with the wet season can isolate sections of the road network and provides a challenge in providing consistent road freight delivery.